Introduction to protein biotechnology

LCHM2170  2018-2019  Louvain-la-Neuve

Introduction to protein biotechnology
3.0 credits
22.5 h + 7.5 h
1q

Teacher(s)
Morsomme Pierre ; Soumillion Patrice ;
Language
Français
Main themes
1. Gene manipulation: recovery, cloning, modification, transfer and characterisation. 2. Gene expression: vectors, expression in bacteria, yeasts, plants, insect and mammalian cells, production of monoclonal antibodies. 3. Protein improvement: genetic engineering, directed evolution and chemical stabilisation. All the underlying techniques will be briefly explained.
Aims
The student will get to know the field of protein biotechnology for which the interactions with chemistry are continuously growing, especially in bio-pharmacy. He will learn the notions of molecular biology and genetic engineering that are useful with regard to the production and improvement of proteins. Another objective is also to acquire the vocabulary associated with these notions so that the student will later be able to interact with the experts of that field.

The contribution of this Teaching Unit to the development and command of the skills and learning outcomes of the programme(s) can be accessed at the end of this sheet, in the section entitled “Programmes/courses offering this Teaching Unit”.

Content
1. Gene manipulation: recovery, cloning, modification, transfer and characterisation. 2. Gene expression: vectors, expression in bacteria, yeasts, plants, insect and mammalian cells, production of monoclonal antibodies. 3. Protein improvement: genetic engineering, directed evolution and chemical stabilisation. All the underlying techniques will be briefly explained.
Teaching methods

Ex cathedra lectures

Evaluation methods

Written exam

Online resources

All documents are proposed via Moodle

Faculty or entity


Programmes / formations proposant cette unité d'enseignement (UE)

Program title
Sigle
Credits
Prerequisites
Aims
Master [120] in Chemical and Materials Engineering
3
-

Master [120] in Chemistry
3
-

Master [60] in Chemistry
3
-