22.5 h + 7.5 h
1. Basic definitions 2. Ionization modes 3. Analyzers 4. Chromatographic couplings 5. Spectral data interpretation 6. Introduction to the identification and sequencing of proteins and peptides by mass spectrometry.
At the end of this learning unit, the student is able to :
|1||This course covers technical aspects of mass spectrometry and interpretation of spectral data.|
After a brief survey of the basic definitions, the various mode of ionization (EI, CI, FAB, ESI, APCI, APPI, DESI, DAPCI, DAPPI) will be described in details. The different analyzers (Quad, triple Quad, Traps, TOF, Orbitrap, FTICR) will be presented together with their possible scanning modes and their combinations. Couplings with Gc and HPLC will be presented. The interpretation of spectral data will first emphasize the differences between low resolution and low accuracy versus high resolution and high accuracy data. The importance of the isotopic cluster will be demonstrated. The principal rules of fragmentation of radical-cations will be presented together with some basic rules for the fragmentation of ions with even-number of electrons. Selected examples and exercises will be explicitly studied in this part of the course. At the end of the course, several examples of the utility of mass spectrometry for different domains will be seen through the presentations of the students.
The participation to all the activities of the course is mandatory to validate the course and be allowed to present the exam. The exam consist in a personnel work based on the scientific literature to be presented to all the other students following the course.
Prerequisite: - Basic knowledge of chemistry and physics - CHM1251C course.
A copy of the slides has been deposited on Moodle
- slides sur moodle
Faculty or entity