Histology and pathological anatomy workshop

Teacher(s)

Guiot Yves; Pierreux Christophe (coordinator); Van Bockstal Mieke;

Language

French

Prerequisites

The prerequisite(s) for this Teaching Unit (Unité d’enseignement – UE) for the programmes/courses that offer this Teaching Unit are specified at the end of this sheet.

Main themes

Theory about morphological methods used to investigate biological methods
and practical training to address an exemplative problem in one week

Learning outcomes

At the end of this learning unit, the student is able to :
1	·           To gain theoretical and practical knowledge of histological and immunohistological techniques, and theoretical notions of in situ hybridization. ·           To solve a biological problem by using morphological methods.

Content

·           Theory :
Histological techniques : frozen sections, paraffin sections, synthetic resin embedding
Immunohistochemical techniques
In situ hybridization
·           Practical :
Mouse dissection and tissues sampling
Preparation of buffers and fixative solutions
Tissue freezing or fixation in formalin
Frozen sections (demonstration)
Immunohistochemistry on frozen sections (demonstration)
Paraffin embedding
Paraffin sections
H&E and PAS staining
Immunohistochemistry on paraffin sections
Supervised microscopic analysis (video screening)

Evaluation methods

Evaluation will be made by oral examination with presentation of the histological section at the microscope and discussion about the report.

Other information

Students will be supervised by PhD students during the training.
Students will have to make a report of the study, resembling a scientific article. They will be paired for this report (one student investigating the control animal, the other one the test animal).

Teaching materials

• Supports du cours disponibles sur Moodle

Faculty or entity

> SBIM