The principle of ApoTome structured illumination fluorescence microscopy is based on the introduction of a grid in the field of the light source and its projection on the sample to be imaged. This projection is used to define the focal plane and to subtract the "unwanted" signal out of this focal plane by means of specific algorithms integrated within acquisition software. It is in this way possible to obtain images in optical sections and to reconstruct thicker samples in 3 dimensions, with the acquisition of successive optical sections (z-stack).

User guide