Technical tips

2IP

 

Preparation of cells and tissues for image acquisition: basics & tips

Antigen retrieval solutions

- Citrate (pH5.7)

Stock solution (10x)  
Citric acid 3.78 g
Sodium citrate dihydrate (C6H5Na3O7.2H20) 24.12 g 
dist H2O ad 1 L
Adjust to pH 5.7  
Store 20 ml aliquots at -20°C  
Working solution  
Stock solution 20 ml
dist H2O 180 ml
Triton X-100 20% 540 µl

- EDTA (1 mM, pH 8,0):    

EDTA (C10H14N2O8Na2.2H2O) 0.372 g
dist H2O ad 1 L
Adjust to pH 8.0  
Store at 4°C  
Extemporaneously:                         Triton X-100 20%                        540 µl

- Tris-EDTA (10 mM Tris/1 mM EDTA, pH 9.0):

Tris Base (C4H11NO3) 12.1 g
EDTA (C10H14N2O8Na2.2H2O) 3.7 g
dist H2O ad 1 L
Adjust to pH 9.0  
Store at 4°C  
Extemporaneously:                         Triton X-100 20%                        540 µl

 

 

Human Protein Atlas (antibodies + protein and RNA expression profiles)

NordiQC (Immunohistochemical quality control)

BenchSci (antibodies used in publications)

ImmunoHistoChemistry Frequently Asked Questions (library of antibodies used in pathology)

Antibodypedia

Antibody-Adviser (academic forum)

Biocompare

 

UCL spin-off SynAbs  is specialized in the production of: 

control isotypes

monoclonal secondary antibodies: anti-mouse, rat, human, pig, horse, chicken Ig

control Ig (anti-DNP,...)

anti-cytokines antibodies

2016 price list

 

 

 

Based on Jockusch et al. (J Histochem Cytochem 2003 51: 401-404)