Fluorescence Microscopy

2IP

Different types of microscopes can be used to image fluorescent samples.

  • Widefield fluorescence microscopy is the most common and simplest form of fluorescent microscopy, where excitation light illuminates the entire (wide)field of view and all the light emitted by the sample (meaning from the focal plane but also from out-of-focus planes) is then collected by a camera for visualization. This allows fast and sensitive imaging adapted to optimization of staining protocols, fast-moving specimens, samples sensitive to photobleaching or to high content imaging.

 

  • For applications requiring higher spatial resolution (like co-localization studies) and for samples that scatter light extensively or are thicker, superior image quality can be obtained by imaging only the focal plane (at the cost of acquisition time). Among the different existing optical sectioning methods, structured illumination and laser scanning confocal are available at 2IP. 

 

  • Lightsheet microscopy combines resolution of optical sectioning and speed of entire field of view acquisition with a camera and is the most adapted technology for fast 3D imaging of thick samples (made transparent by a tissue optical clearing method).  

AxioImager.z1/ApoTome

Confocal microscopy

Lightsheet microscopy