Sample Preparation

2IP

2IP helps you to prepare your samples for imaging

 

- by entrusting them to our two technicians

- or by using the Histo-lab infrastructure yourself (except for primary antibodies and samples, all equipment and reagents are available) 

 

Proposed services and available equipment:

  • Paraffin sections

  

Microtomes Microm HM 340 E; HM 355 S ER; Microm HM 340 E equiped with Section Transfer System (STS) and Cool-Cut

 

  • Cryosections

cryostat Leica CM1950

  • Histological staining

HE, trichrome de Masson, picro-Sirius red, PAS, Oil red O, rouge Soudan)

  • Chromogenic Immunohistochemistry (IHC-DAB), multiplex immunofluorescence (TSA method)

Leica Bond RXm autostainer (user guide)

 

 

Information and advices

Advice for the design of your immunostainings

How to choose a primary antibody? 

Standard immunostaining protocols

Standard histological staining protocols

 

Interested by these services? Feel free to contact us