Whole Slide Imaging (WSI) technology (or virtual microscopy) consists of scanning histological sections at high resolution, in brightfield or in fluorescence, in order to obtain an overview of the biological tissue. In addition to the global vision of the histological sections, it is possible to move on the virtual slide, to adjust the brightness and to zoom in on areas of interest with high definition.
The main advantage over traditional microscopy images (pathologists or researchers selecting fields of interest) is to make the analysis independent of the choices made by the first observer. Furthermore, the comparison of images, for example of different stainings performed on adjacent sections, is greatly facilitated since the images can be placed side by side on the same computer screen and synchronized.
Digital images of whole sections can be viewed and stored, but also shared, thus facilitating collaborative studies, teaching and publication. In addition, the content of digital images can be exploited in a standardized and rapid manner using specific software for quantitative measurement of stained areas, cell number, biomarkers, vascularization, fibrosis, etc.