Cell analysis

Bruxelles Woluwe

 

Cell analysis: FACSCantoII™ and FACSCalibur™

FACSCantoII™

The analyzer BD FACSCantoII™ is one of the most versatile and intuitive flow cytometers thanks to BD FACSDiva™ software that automatically checks the configuration, acquisition and analysis. The automatic and off-line compensation process saves time to researchers. Recorded data can be analyzed in different ways and are easily identified through the hierarchical gates.

User Guide

Specifications

3 lasers, 8 fluorescences :

  • 405nm: Pacific Blue, H450, BV421 / AmCyan, H500, BV510
  • 488nm: FITC, Alexa-488, GFP, CFSE/ PE / PerCP, PE-Cy5.5, 7-AAD, PI / PE-Cy7
  • 633nm: APC / APC-Cy7, APC-H7

 

Applications

 

Training

Each researcher can use the instrument after following the course taught by Davide Brusa or demonstrating proficiency in the use of the same device.

 

Fees

  IREC UCL Private
FACSCantoII   25 €/h   35 €/h on request

 


 

FACSCalibur™

The BD FACSCalibur™ platform allows users to perform cell analysis and four colours immunophenotype. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications.

User guide

Specifications

2 lasers, 4 fluorescences :

  • 488nm: FITC, Alexa-488, GFP, CFSE/ PE / PerCP, PE-Cy5.5, 7-AAD, PI
  • 633nm: APC

Applications

  • Cell cycle (Link1; Link2; Link3)
  • DNA/RNA content
  • Cell proliferation
  • Cell death

 

Training

Each researcher can use the instrument after following the course taught by Davide Brusa or demonstrating proficiency in the use of the same device.

 

Fees

  IREC UCL Private
FACSCalibur   20 €/h   30 €/h on request