Cholesterol-enriched domains and cellular toxicity

Bruxelles Woluwe

The existence of clusters of proteins and lipids and especially, the transient nanometric cholesterol-and sphingolipid-enriched domains, called rafts,are described as signaling platforms for a wide range of cellular responses to stimuli including reactive oxygen species (ROS) generation, inflammatory cytokines expression and cell death. we explored the role of cholesterol and cholesterol-enriched domains for cellular toxicity ofthe potential anticancer drug, the ginsenoside Rh2 and the anti-inflammatory complex budesonide: HPβCD.

Taking benefit from our previous studies investigating the mechanisms involved in nephrotoxicity induced by aminoglycoside antibiotics, we explored thecapacity of new antibiotics to accumulate within the cells and to induce accumulation of undigested lipids within the lysosomes. More recently, we started to explore the mitochondrial alterations induced by oxazolidinone antibiotics.