Lipid domains: a promising target for new antibiotics

Bruxelles Woluwe

With the aim to provide a more comprehensive and biologically relevant picture of the drug-membrane interactions and how the effect of these interactions can modify the biophysical properties of the membranes in relation with pharmacological activities, most of the studies are performed by using cells (bacteria or mammalian cells) and membrane models (supported bilayers, liposomes [SUVs, LUVs; GUVs]) mimicking (i) bacterial and (ii) eukaryotic membranes. In close collaboration, we used a range of complementary methods including AFM, 31P NMR, dynamic light scattering, fluorescence spectroscopy (Laurdan, DPH, TMA-DPH, DHE, calcein, octadecylrhodamine B…) and confocal and electronic microscopy.