Overview of technologies


1D and 2D-PAGE

  • Classical (silver, Colloidal Coomassie blue)
  • Fluorescent protein stains (DIGE using CyTM dyes)
  • Post translational modification specific (eg Pro-Q Diamond)
  • Gel Image Analysis
  • Image scanner Labscan
  • Image Master 2D Platinum
  • Decyder v7.0
  • Spot Excision/Enzymatic Digestion of Proteins

Mass Spectrometric analysis

  • Peptide mass fingerprinting MALDI TOF MS
  • Peptide fragments fingerprint ESI-IT
  • Sequence analysis 4800 MALDI TOF/TOF
  • Post-translation modification analysis-phosphorylation, acetylation
  • iTRAQ and LC-MS/MS workflows for protein identification and relative quantification
  • Bioinformatics
  • Mass spectrometry data analysis
  • Data storage