Response to Berbey et al , Journal of Biological Chemestry, 2009, Oct 29

NIcolas Tajeddine, Nadège Zanou, Monique Van Schoor, Jean Lebacq, Philippe Gailly Laboratory of Cell Physiology, Institute of Neurosciences, Université catholique de Louvain, 55/40 av. Hippocrate, 1200 Brussels, Belgium Contact: Philippe.Gailly

TRPC1 immunolabeling 

Flexor Digitorum Brevis muscle fibers were isolated from WT and TRPC1-deficient mice (1) (upper and lower panels respectively) and fixed and immunolabeled as decribed in (2) with anti-TRPC1 antibody (Chemicon). We observed a striated pattern in both groups (large staining in A-bands and faint staining in M-bands, as shown on the profile curves, performed along the red line). Similar results were obtained on longitudinal sections of paraffin-embedded EDL muscles (not shown). 

References. 1. Dietrich, A., Kalwa, H., Storch, U., Mederos, Y. S. M., Salanova, B., Pinkenburg, O., Dubrovska, G., Essin, K., Gollasch, M., Birnbaumer, L., and Gudermann, T. (2007) Pflugers Arch 455, 465-477 2. Berbey, C., Weiss, N., Legrand, C., and Allard, B. (2009) J Biol Chem