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- Delia Hoffmann
Delia Hoffmann
Chargée de recherche FNRS
Our team (ContrasT Team), under the supervision of Prof. Ir. Greet Kerckhofs, focuses on contrast-enhanced X-ray microfocus computed tomography (CECT) as an emerging 3D histology tool that enables to visualize and structurally analyze multiple biological tissues in full 3D at the same time. For now, sectioning followed by colorimetric or fluorescent staining is still the gold standard for histological analysis of tissues. However, it is well known that this technique has limitations with regards to the 3D microstructural assessment of biological tissues and their constituents, and to the quantification of their spatial interactions. It is also a destructive, labor-intensive technique, and is mostly restricted to 2D sections. Using contrast-enhancing staining agents (CESAs), CECT can tackle these shortcomings, allowing soft tissue visualization in full 3D. The ContrasT Team attempts to develop, optimize and validate novel CESAs and staining protocols. Within this context, my postdoc project focuses on the development of antibody-based CECT staining protocols for whole tissues, similar to immunohistochemical stainings on tissue sections.
Videos and more : https://contrast-team.be/
2023
Vanderroost, Juliette ; Avalosse, Noémie ; Mohammed, Danahe ; Hoffmann, Delia ; Henriet, Patrick ; Pierreux, Christophe ; Alsteens, David ; Tyteca, Donatienne. Cholesterol and sphingomyelin polarize at the leading edge of migrating myoblasts and involve their clustering in submicrometric domains.. In: Biomolecules, (2023) (Accepté/Sous presse).
2021
Klaessens, Simon ; Stroobant, Vincent ; Hoffmann, Delia ; Gyrd-Hansen, Mads ; Pilotte, Luc ; Vigneron, Nathalie ; De Plaen, Etienne ; Van den Eynde, Benoît. Tryptophanemia is controlled by a tryptophan-sensing mechanism ubiquitinating tryptophan 2,3-dioxygenase. In: Proceedings of the National Academy of Sciences, Vol. 118, no.23, p. e2022447118 (2021). doi:10.1073/pnas.2022447118.
2020
Schramme, Florence ; Crosignani, Stefano ; Frederix, Kim ; Hoffmann, Delia ; Pilotte, Luc ; Stroobant, Vincent ; Preillon, Julie ; Driessens, Gregory ; Van den Eynde, Benoît. Inhibition of Tryptophan-Dioxygenase Activity Increases the Antitumor Efficacy of Immune Checkpoint Inhibitors.. In: Cancer immunology research, Vol. 8, no. 1, p. 32-45 (2020). doi:10.1158/2326-6066.CIR-19-0041.
2020
Hoffmann, Delia ; Dvorakova, Tereza ; Stroobant, Vincent ; Bouzin, Caroline ; Daumerie, Aurélie ; Solvay, Marie ; Klaessens, Simon ; Letellier, Marie-Claire ; Renauld, Jean-Christophe ; van Baren, Nicolas ; Lelotte, Julie ; Marbaix, Etienne ; Van den Eynde, Benoît. Tryptophan 2,3-Dioxygenase Expression Identified in Human Hepatocellular Carcinoma Cells and in Intratumoral Pericytes of Most Cancers.. In: Cancer immunology research, Vol. 8, no. 1, p. 19-31 (2020). doi:10.1158/2326-6066.CIR-19-0040.
2020
Hoffmann, Delia ; Dvorakova, Tereza ; Schramme, Florence ; Stroobant, Vincent ; Van den Eynde, Benoît. Tryptophan 2,3-Dioxygenase Expression Identified in Murine Decidual Stromal Cells Is Not Essential for Feto-Maternal Tolerance.. In: Frontiers in immunology, Vol. 11, p. 601759 (2020). doi:10.3389/fimmu.2020.601759.
2019
Hoffmann, Delia ; Pilotte, Luc ; Stroobant, Vincent ; Van den Eynde, Benoît. Induction of tryptophan 2,3-dioxygenase expression in human monocytic leukemia/lymphoma cell lines THP-1 and U937.. In: International journal of tryptophan research : IJTR, Vol. 12, no.1, p. 1178646919891736 (2019). doi:10.1177/1178646919891736.
2017
Hennequart, Marc ; Pilotte, Luc ; Cane, Stefania ; Hoffmann, Delia ; Stroobant, Vincent ; De Plaen, Etienne ; Van den Eynde, Benoît. Constitutive IDO1 Expression in Human Tumors Is Driven by Cyclooxygenase-2 and Mediates Intrinsic Immune Resistance. In: Cancer Immunology Research, Vol. 5, no. 8, p. 695-709 (2017). doi:10.1158/2326-6066.cir-16-0400.
2015
Tyteca, Donatienne ; Nishino, Tomoya ; Debaix, Huguette ; Van Der Smissen, Patrick ; N'Kuli, Francisca ; Hoffmann, Delia ; Cnops, Yvette ; Rabolli, Virginie ; van Loo, Geert ; Beyaert, Rudi ; Bradl, Monika ; Huaux, François ; Devuyst, Olivier ; Courtoy, Pierre J.. Regulation of Macrophage Motility by the Water Channel Aquaporin-1: Crucial Role of M0/M2 Phenotype Switch. In: PLoS One, Vol. 10, no. 2, p. e0117398 [1-23] (2015). doi:10.1371/journal.pone.0117398.
2022
Tim Balcaen ; Catherine Piens ; Hoffmann, Delia ; Fabien Chauveau ; Wim De Borggraeve ; Kerckhofs, Greet. Revealing the murine brain anatomy by contrast-enhanced computed tomography – a screening study. 6th Symposium on X-ray Computed Tomography (Kasteelpark Arenberg 10, 3001 Leuven, du 24/05/2022 au 25/05/2022).
2021
Balcaen, Tim ; MARCHAL, Mathilde ; El Aazmani, Walid ; Hoffmann, Delia ; Wim De Borggraeve ; Kerckhofs, Greet. Design of experiments to evaluate the staining properties of polyoxometalates for contrast-enhanced microCT of aorta tissue. BSTE 2021 – 8th Belgian Symposium on Tissue Engineering ( Louvain-la-Neuve, du 12/11/2021 au 13/11/2021).
2021
Hoffmann, Delia ; El Aazmani, Walid ; Engelen, Lara ; Kerckhofs, Greet. Virtual 3D histological analysis of soft tissues by contrast-enhanced microfocus computed tomography: screening contrast-enhancing staining agents. ToScA - Tomography for Scientific Advancement.
2021
Hoffmann, Delia ; El Aazmani, Walid ; Engelen, Lara ; Balcaen, Tim ; Vangrunderbeeck, Sarah ; Kerckhofs, Greet. Virtual 3D histological analysis of soft tissues by contrast-enhanced microfocus computed tomography: screening contrast-enhancing staining agents. BSTE - Belgian Symposium on Tissue Engineering.
2019
Hoffmann, Delia ; Vangrunderbeeck, Sarah ; Maistriaux, Louis ; Tohala Pulgarin, Karem Paulette ; Fievé, Lies ; Duisit, Jérôme ; Lengelé, Benoît ; Behets Wydemans, Catherine ; Kerckhofs, Greet. Contrast-enhanced microfocus computed tomography of body parts and organs: screening and validation of contrast agents. BSTE 2019 (Hasselt, du 14/11/2019 au 15/11/2019).
2019
Hoffmann, Delia. Expression and potential immunosuppressive role of tryptophan 2,3-dioxygenase in tumours and placental tissues, prom. : Van den Eynde, Benoît, 27/05/2019.