Proteomic & protein analysis by mass spectrometry (MASSPROT)
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Picture credit : Yvan Flamant - MASSPROT Woluwé
Technical and scientific expertise
- Proteomics: scientific and technical advice and support at all stages of sample preparation and mass spectrometry experiments in areas such as:
- identification and expression changes of proteins
- identification and quantification of proteins from complex samples
- localisation of post-translational modifications even in complex samples
- analysis of intact proteins
- Bioinformatics: advice and data analysis using appropriate IT tools and software
Main equipment
Mass spectrometry, chromatography, two-dimensional electrophoresis techniques:
- Orbitrap Fusion Lumos Tribrid Mass spectrometer (Thermo Scientific)
- Orbitrap Exploris 240 Mass spectrometer (Thermo Scientific)
- Synapt G2si spectrometer (Waters)
- Acquity MClass nano UPLC System (Waters)
- Ultimate 3000 RSLN nano HPLC System (Thermo Scientific)
Get an overview of the platform’s facilities via a virtual tour.
Applications
- Measurement of intact protein masses for quality control
- Identification of purified proteins on gel
- De novo sequencing of peptides for protein identification
- Quantification of differential protein expression
- Identification of covalent modification sites by peptide fragmentation/dissociation
Services available to
- UCLouvain students and researchers
- Non-UCLouvain parties