Proteomic & protein analysis by mass spectrometry (MASSPROT)

PFTPLUS

SYNAPT G2-Si high definition mass spectrometer (WATERS) coupled to the NanoACQUITY UPLC MClass system (WATERS)   Orbitrap Fusion Lumos (IET) coupled with RSLC nanoHPLC Ultimate 3000 (THERMOFISCHER SCIENTIFIC)   Orbitrap Fusion Lumos Nano-Spray Source (IET)  

Technical and scientific expertise

  • Proteomics: scientific and technical advice and support at all stages of sample preparation and mass spectrometry experiments in areas such as:
    • identification and expression changes of proteins
    • identification and quantification of proteins from complex samples
    • localisation of post-translational modifications even in complex samples
    • analysis of intact proteins
  • Bioinformatics: advice and data analysis using appropriate IT tools and software

Main equipment

Mass spectrometry, chromatography, two-dimensional electrophoresis techniques:

  • Orbitrap Fusion Lumos Tribrid Mass spectrometer (Thermo Scientific)
  • Orbitrap Exploris 240 Mass spectrometer (Thermo Scientific)
  • Synapt G2si spectrometer (Waters)
  • Acquity MClass nano UPLC System (Waters)
  • Ultimate 3000 RSLN nano HPLC System (Thermo Scientific)

Get an overview of the platform’s facilities via a virtual tour.

Applications

  • Measurement of intact protein masses for quality control
  • Identification of purified proteins on gel
  • De novo sequencing of peptides for protein identification
  • Quantification of differential protein expression
  • Identification of covalent modification sites by peptide fragmentation/dissociation

Services available to

  • UCLouvain students and researchers
  • Non-UCLouvain parties

News

HDx-MS technology now available at MASSPROT (De Duve...

With the acquisition of a new instrument dedicated to deuterium-hydrogen exchange, funded by the Fondation Contre le Cancer, the MASSPROT mass spectrometry platform is moving towards the study...

Mass spectrometry to detect Alzheimer's disease

The MASSPROT platform is contributing to the characterisation of proteins involved in Alzheimer's disease. While today only an autopsy can determine with certainty whether a patient is...

Innovate in your company with the technology platforms...

Boost innovation in your company with the technology platforms of UCLouvain Did you know? Technology platforms give you access to expertise, equipment and infrastructures at the cutting...