June 01, 2018
12:50
Seminar Room ISV
Frank Hollmann: Are peroxygenases the new P450s? Scope and current challenges of peroxygenases for selective oxyfunctionalisation chemistry
Peroxygenases are experiencing a renewed interest as catalysts for selective oxyfunctionalisation chemistry. Peroxygenases are promising alternatives to the well-known P450 monooxygenases due to the significantly simpler regeneration scheme.
Comparison of P450-monooxygenases and peroxygenases with respect to the regeneration of the catalytically Compound I.
New peroxygenases enable selective hydroxylation of non-activated C-H bonds in alkanes and aromatics, epoxidation and heteroatom oxygenation. Yet, to assess the full scope of this exciting enzyme class a range of challenges need to be met: (1) more enzyme (variants) with tailored properties need to be identified/evolved; (2) new in situ H2O2-generation systems to minimise oxidative enzyme inactivation need to be established; (3) peroxygenase-reactions in non-aqueous media need to be established.
These issues together with some promising solutions will be discussed.