Publications

2017

  • D. Alsteens, H.E. Gaub, R. Newton, M. Pfreundschuh, C. Gerber, D.J. Müller, Atomic force microscopy based characterization and design of biointerfaces, Nat. Rev. Mater., 2 (2017).
  • Y.F. Dufrêne, T. Ando, R. Garcia, D. Alsteens, D. Martinez-Martin, A. Engel, C. Gerber, D.J.  Müller, Imaging modes of atomic force microscopy for application in molecular and cell biology, Nat. Nanotechnol., 12 (2017), 395-307.
  • D. Alsteens, D.J. Müller, Y.F. Dufrêne, Multiparametric atomic force microscopy imaging of biomolecular and cellular systems, Acc. Chem. Res., 50 (2017), 924-931.
  • D. Alsteens, R. Newton, R. Schubert, D Martinez-Martin, M. Delguste, B. Roska, DJ Müller, Nanomechanical mapping of first binding steps of a virus to animal cells, Nat. Nanotechnol., 12 (2017), 177-183.

2016

  • P. Herman, C. Valotteau, G. Pietrocola, S. Rindi, D. Alsteens, T.J. Foster, P. Speziale, Y.F. Dufrêne, Mechanical Strength and Inhibition of the Staphylococcus aureus Collagen-Binding Protein Cna, mBio., 7 (2016).
  • M. Kragel, R.  Schubert, H. Karsjens , S. Otter, B. Bartosinska, K.  Jeruschke, J.  Weiss, C. Chen, D.  Alsteens, O. Kuss, S. Speier, D. Eberhard, D.J. Müller, E. Lammert, The biomechanical properties of an epithelial tissue determine the location of its vasculature, Nat. Commun., 7 (2016).
  • D. Alsteens, Study of membrane receptors by atomic force microscopy, Med./Sci., 5 (2016), 433-435.

2015

  • M. Pfreundschuh, D. Alsteens, R. Wieneke, C. Zhang, S. Coughlin, R. Tampé, B. Kobilka, D.J. Müller, Imaging single G-protein coupled receptors while quantifying their binding to two different ligands, Nat. Commun., 6 (2015).
  • D. Alsteens, M. Pfreundschuh, C. Zhang, P. Spoerri, S. Coughlin, B. Kobilka, D.J. Müller, Imaging of G-protein coupled receptors while quantifying their ligand-binding free energy landscape, Nat. Methods., 12 (2015), 845-851.
  • R. Petrosyan, C. Bippes, S.Walheim, D. Harder, D. Fotiadis, T. Schimmel, D. Alsteens, D.J. Müller, Single-molecule force spectroscopy of membrane proteins from membranes freely spanning across nanoscopic pores, Nano Lett., 15 (2015), 3624-3633.
  • S. El-Kirat-Chatel, A. Beaussart, S. Derclaye, D.Alsteens, S. Kucharikova, P. Van Dijck, Yves Dufrêne, Force nanoscopy of hydrophobic interactions in the fungal pathogen Candida glabrata, ACS Nano., 9 (2015), 1648-1655.
  • D. Alsteens, S. Tay, D.J. Müller, Towards high-throughput biomechanical phenotyping of single molecules (News and Views), Nat. Methods., 12 (2015), 45-46.

2014

  • M. Pfreundschuh, D. Alsteens, M. Hilbert, M.O. Steinmetz, D.J. Müller, Localizing chemical groups while imaging single native proteins by high-resolution AFM, Nano Lett., 14 (2014), 2957-2964.
  • E. Dague, G. Genet, V. Lachaize, C. Guilbeau-Frugier, J. Fauconnier, C. Mias, B. Payre, L. Chopinet, D. Alsteens, S. Kasas, C. Severac, J. Thireau, C. Heymes, B. Honton, A. Lacampagne, A. Pathak, J.M. Senard, C. Gales , Atomic force and electron microscopic-based study of sarcolemmal surface of living cardiomyocytes unveils unexpected mitochondrial shift in heart failure, J. Mol. Cell. Cardiol., 74 (2014), 162-172.
  • Beaussart, S. El-Kirat-Chatel, R.M.A Sullan, D. Alsteens, P. Herman, S. Derclaye, Y.F. Dufrêne, Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy, Nat. Protocols., 9 (2014), 1049-1055.

2013

  • D. Alsteens, H. Trabelsi, P. Soumillion, Y.F. Dufrêne, Multiparametric atomic force microscopy imaging of single bacteriophage extruding from living bacteria, Nat. Commun., 4 (2013).
  • D. Alsteens, P. Van Dijck, P.N. Lipke, Y.F. Dufrêne , Quantifying the forces driving cell-cell adhesion in a fungal pathogen, Langmuir., 29 (2013), 13473-13480.
  • D. Alsteens, A. Beaussart, S. El-Kirat-Chatel, R.M.A. Sullan, Y.F. Dufrêne, Atomic force microscopy: a new look at pathogens, PLoS Pathogens., 9 (2013).
  • Y.F. Dufrêne, D. Martinez-Martin, I. Medalsy, D. Alsteens, D.J. Müller, Multiparametric imaging of biological systems by force-distance curve-based AFM, Nat. Methods., 10 (2013), 847-854.
  • D.Alsteens, N. Martinez, M. Jamin, F. Jacob-Dubuisson, Sequential unfolding of beta helical protein by single-molecule atomic force microscopy, PLoS One., 8 (2013).
  • D. Alsteens, V. Aimanianda, P. Hegde, S. Pire, R. Beau, J. Bayry, J.-P. Latge, Y.F. Dufrêne, Unraveling the nanoscale surface properties of Chitin synthase mutants of Aspergillus fumigatus and their biological implications, Biophys. J., 105 (2013), 320–327.
  • D. Alsteens, A. Beaussart, S. Derclaye, S. El-Kirat-Chatel, H.R. Park, P.N. Lipke, Y.F. Dufrêne, Single-cell force spectroscopy of Als-mediated fungal adhesion, Analytical Methods., 5 (2013), 3657-3662.
  • S. El-Kirat-Chatel, A. Beaussart, D. Alsteens, A. Sarazin, T. Jouault, Y.F. Dufrêne, Single-molecule analysis of the major glycopolymers of pathogenic and non-pathogenic yeast cells, Nanoscale., 5 (2013), 4855-4863.
  • D. Alsteens, Role of the amyloid region in the formation and propagation of Als adhesive nanodomains on Candida albicans, Proceedings of SPIE., 8590 (2013).
  • A. Beaussart, S. El-Kirat-Chatel, P. Herman, D. Alsteens, J. Mahillon, P. Hols, Y.F. Dufrêne, Single-cell force spectroscopy of probiotic bacteria, Biophys. J., 104 (2013), 1886-1892.
  • Tripathi, A. Beaussart, D. Alsteens, V. Dupres, I. Claes I. Von Ossowski, W.M. De Vos, A. Palva, S. Lebeer, J. Vanderleyden, Y.F. Dufrêne, Adhesion and nanomechanics of pili from the probiotic Lactobacillus rhamnosus GG, ACS Nano., 7 (2013), 3685-3697.
  • S. El-Kirat-Chatel, A. Beaussart, D. Alsteens, D.N. Jackson, P.N. Lipke, Y.F. Dufrêne, Nanoscale analysis of caspofungin-induced cell surface remodeling in Candida albicans, Nanoscale., 5 (2013), 1105-1115.

2012

  • D. Alsteens, V. Dupres, S. Yunus, J.P. Latgé, J.J. Heinisch, Y.F. Dufrêne,  High-resolution imaging of chemical and biological sites on living cells using peak force tapping atomic force microscopy, Langmuir., 28 (2012), 16738-16744.
  • A. Beaussart, D. Alsteens, S. El-Kirat-Chatel, P.N. Lipke, S. Kucharíková, P. Van Dijck, Y.F. Dufrêne, Single-molecule imaging and functional analysis of Als adhesins and mannans during Candida albicans morphogenesis, ACS Nano., 12 (2012), 10950-10964.
  • C.Jimeńez-Ortigosa, V. Aimanianda, L. Muszkieta, I. Mouyna, D. Alsteens, S. Pire, R. Beau, S. Krappmann, A. Beauvais, Y.F. Dufrêne, C. Roncero, J.P. Latgé, Chitin synthases with a myosin motor-like domain control the resistance of Aspergillus fumigatus to echinocandins, Antmicrob. Agents Ch., 56 (2012), 6121-6131.
  • J.J Heinisch, P.N. Lipke, A. Beaussart, S. El Kirat Chatel, V. Dupres, D. Alsteens, Y.F. Dufrêne, Atomic force microscopy–looking at mechanosensors on the cell surface, J. Cell Sci., 125 (2012), 4189-4195.
  • D. Alsteens, C.B. Ramsook, P.N. Lipke, Y.F. Dufrêne, Unzipping a functional microbial amyloid, ACS Nano., 6 (2012), 7703-7711.
  • D. Alsteens, Microbial cells analysis by atomic force microscopy, Methods in Enzymology., 506 (2012), 3-17.
  • P.N. Lipke, M.C. Garcia, D. Alsteens, C.B. Ramsook, S.A. Klotz, Y.F Dufrêne, Strenghtening relationships: Amyloids create adhesion nanodomains in yeasts, Trends Microbiol., 20 (2012), 59-65.

2011

  • M.C. Garcia, J.T. Lee, C.B. Ramsook, D. Alsteens, Y.F. Dufrêne, P.N. Lipke,  A role for amyloid in cell aggregation and biofilm formation, PLOS One., 6 (2011).
  • M. Ouberai, F. El Garch, A. Bussiere, M. Riou, D. Alsteens, L. Lins, I. Baussanne, Y. F. Dufrêne, R. Brasseur, J.L. Decout, M.P. Mingeot-Leclercq, The Pseudomonas aeruginosa membranes : a target for a new amphiphilic aminoglycoside derivative?, Biochim. Biophys. Acta-Biomembr., 6 (2011), 1716-1727.
  • D. Alsteens, V. Dupres, G. Andre, Y.F. Dufrêne, Frontiers in microbial nanoscopy, Nanomedicine., 6 (2011), 395-403.

2010

  • D. Alsteens, M.C. Garcia, P.N. Lipke, Y.F. Dufrêne, Force-induced formation and propagation of adhesion nanodomains in living fungal cells, Proc. Natl. Acad. Sci. USA., 107 (2010), 20744-20749.
  • V. Dupres, D. Alsteens, G. Andre, Y.F. Dufrêne,  Microbial nanoscopy: a closer look at microbial cell surfaces, Trends Microbiol.,  18 (2010), 397-405.
  • J.J. Heinisch, V. Dupres, D. Alsteens, Y.F. Dufrêne, Measurement of the mechanical behavior of yeast membrane sensors using single-molecule atomic force microscopy, Nat. Protoc., 5 (2010), 670-673.

2009

  • D. Alsteens, E. Dague, C. Verbelen, G. Andre, V. Dupres, Y.F. Dufrêne, Nanoscale imaging of microbial pathogens using atomic force microscopy, WIREs Nanomed. Nanobiotechnol., 1 (2009), 68-180.
  • D.J. Müller, M. Krieg, D. Alsteens, Y.F. Dufrêne, New frontiers in atomic force microscopy: analyzing interactions from single-molecules to cells, Curr. Opin. in Biotechnol., 20 (2009), 4-13.
  • D.J. Müller, J. Helenius, D. Alsteens, Y.F. Dufrêne, Force probing surfaces of living cells to molecular resolution. Nat. Chem. Biol., 5 (2009), 383-390.
  • V. Dupres, D. Alsteens, K. Pauwels, Y.F. Dufrêne, In vivo imaging of S-layer nanoarrays on Corynebacterium glutamicum, Langmuir., 25 (2009), 9653-9655.
  • V. Dupres, D. Alsteens, G. Andre, C. Verbelen, Y.F. Dufrêne, Fishing single molecules on live cells, Nanotoday., 4 (2009), 262-268.
  • V. Dupres, D. Alsteens, S. Wilk, B. Hansen, J.J. Heinisch, Y.F. Dufrêne, The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo, Nat. Chem. Biol., 5 (2009), 857-862.
  • D. Alsteens, E. Pesavento, G. Cheuvart, V. Dupres, H. Trabelsi, P. Soumillion, Y.F. Dufrêne, Controlled manipulation of bacteriophages using single-virus force spectroscopy, ACS Nano., 3 (2009), 3063-3068.
  • D. Alsteens, V. Dupres, S.A. Klotz, N.K. Gaur, P.N. Lipke, Y.F. Dufrêne, Unfolding individual Als5p adhesion proteins on live cells, ACS Nano., 3 (2009), 1677-1682.
  • G. Francius, D. Alsteens, V. Dupres, S. Lebeer, S. De Keersmaecker, J. Vanderleyden, H.J. Gruber, Y.F. Dufrêne, Stretching polysaccharides on live cells using single molecule force spectroscopy, Nat. Protoc., 4 (2009), 939-946.
  • C. Verbelen, N. Christiaens, D. Alsteens, V. Dupres, A.R. Baulard, Y.F. Dufrêne, Molecular mapping of lipoarabinomannans on mycobacteria, Langmuir., 25 (2009), 4324-4327.

2008

  • E. Dague, D. Alsteens, J.-P. Latgé, Y.F. Dufrêne, High-resolution cell surface dynamics of germinating Aspergillus fumigatus conidia, Biophys. J., 94 (2008), 656-660.
  • G. Francius, S. Lebeer, D. Alsteens, L. Wilding, H. J. Gruber, P. Hols, S. De Keersmaecker, J. Vanderleyden, Y. Dufrêne,  Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria, ACS Nano., 2 (2008), 1921-1929.
  • D. Alsteens, C. Verbelen, E. Dague, D. Raze, A.R. Baulard, Y.F. Dufrêne, Organization of the mycobacterial cell wall: a nanoscale view, Pflüg. Arch. Eur. J. Phy., 456 (2008), 117-125.
  • D. Alsteens, V. Dupres, K. Mc Evoy, L. Wildling, H.J. Gruber, Y.F. Dufrêne, Structure, cell wall elasticity and polysaccharide properties of living yeast cells, as probed by AFM, Nanotechnol., 19 (2008).

2007

  • E. Dague, Y. Gilbert, C. Verbelen, G. Andre, D. Alsteens, Y.F. Dufrêne, Towards a nanoscale view of fungal surfaces, Yeast., 24 (2007), 229-237.
  • D. Alsteens, E. Dague, C. Verbelen, G. Andre, G. Francius, Y.F. Dufrêne, Nanomicrobiology, Nanoscale Res. Lett., 2 (2007), 365-372.
  • E. Dague, D. Alsteens, J.P. Latgé, C. Verbelen, D. Raze, A.R. Baulard, Y.F. Dufrêne, Chemical force microscopy of single live cells, Nano Lett., 7 (2007), 3026-3030.
  • D. Alsteens, E., Dague, P.G., Rouxhet, A.R., Baulard, Y.F. Dufrêne, Direct measurement of hydrophobic forces on cell surfaces using AFM, Langmuir., 23 (2007), 11977-11979.