Flow Cytometry platform (CYTOFLUX)

PFTPLUS

Sample preparation: GentleMACS™ Octo Dissociator with Heaters Cell analysis: FACSCalibur™ Cell sorting: FACSAriaIII™ Data analysis: FACSDiva™

Technical and scientific expertise

  • Experiment design
  • Sample preparation and cell handling with researchers
  • Panel design
  • Sample acquisition
  • Cell sorting experiments
  • Data interpretation
  • FACS training to access the platform

Protocols are being developed for surface, intracellular, intranuclear (immune) staining and functional analysis in murine and human species.

Main equipment

The CYTOFLUX Platform is equipped with two analysers and a cell sorter for cell separation as well as a tissue dissociator to obtain a single cell suspension before cell staining.

  • GentleMACS, dissociator with heaters (new acquisition in 2019)
  • FACSCalibur, analyser, two lasers, four fluorescences ;
  • FACSCantoII, analyser, three lasers, eight fluorescences ;
  • FACSAriaIII, cell sorter, four lasers, sixteen fluorescences ;
  • Analysis station equipped with FACSDiva, FlowJo, FACSkin and R software.

Get an overview of CYTOFLUX Platform equipment via a virtual tour.

Applications

  • Immunophenotyping and detection of rare events
  • TCR repertoire
  • Signal transduction (PhosFlow)
  • Cell cycle
  • DNA/RNA content
  • Cell proliferation
  • Cell death
  • Alteration of mitochondria and cell membrane potential
  • mRNA and microRNA detection
  • Exosome and microvesicle detection

Services available to

  • UCLouvain students and researchers
  • Non-UCLouvain parties

News

CytoFlux: a new equipment to detect platelets, bacteria...

Installed in October 2022 at the Flow Cytometry and Cell Sorting Platform of IREC (CytoFlux), Agilent's new NovoCyte Quanteon is one of the most advanced and highly performing equipment in the...