Technical and scientific expertise
- 13C metabolomics studies
- Identification of peptides by mass spectrometry
Main equipment
- Hypoxia station (cell culture at 0.1-21% O2)
- Bioenergetic analyser
- Microdialysis
- Two-dimensional (2D) gel run platform
- Associated equipment for 2D-DIGE (Two-Dimensional DIfference Gel Electrophoresis) studies
- Microscale liquid chromatography
- Multiplex immunoassay system
Applications
- Real-time measurements of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR)
- Metabolite monitoring: e.g. lactate, pyruvate, urea, glutamate
- Monitoring of radiolabelled nutrient/metabolite flux: e.g. glucose, lactate, pyruvate, palmitate
Services available to
- UCLouvain students, clinicians and researchers
- In the context of UCLouvain partners