September 18, 2019
How strong is the fibrinogen bridge between staphylococcal surface protein ClfA and endothelial cell integrin aVb3 ?
Binding of the Staphylococcus aureus surface protein clumping factor A (ClfA) to endothelial cell integrin aVb3 plays a crucial role during sepsis, by causing endothelial cell apoptosis and loss of barrier integrity. ClfA uses the blood plasma protein fibrinogen (Fg) to bind to aVb3, but how this is achieved at the molecular level is not known. In a Nano Lett paper we demonstrate that the ClfA-Fg-aVb3 ternary complex is extremely stable, being able to sustain forces (∼800 pN) that are much stronger than those of classical bonds between integrins and the Arg-Gly-Asp (RGD) tripeptide sequence (∼100 pN). Our experiments favor a binding mechanism involving the extraordinary elasticity of Fg. In the absence of mechanical stress, RGD sequences in the Aa chains mediate weak binding to aVb3, whereas under high mechanical stress, exposure of cryptic Aa chain RGD sequences leads to extremely strong binding to the integrin.
May 26, 2019
Owing to David and the team we hosted ISPM 2019 which was a real success !
April 30, 2019
Fluidic force microscopy captures amyloid bonds between yeast cells
Congrats to Jérôme et al., including P. Lipke (New York Brooklyn College), for measuring the forces driving Als5-mediated intercellular adhesion in Candida albicans, using an innovative fluidic force microscopy platform (Nano Lett). The results point to a model whereby amyloid-like β-sheet interactions play a dual role in cell-cell adhesion, that is, in formation of adhesin nanoclusters ( cis-interactions) and in homophilic bonding between amyloid sequences on opposing cells ( trans-interactions). Because potential amyloid-forming sequences are found in many microbial adhesins, we speculate that this novel mechanism of amyloid-based homophilic adhesion might be widespread and could represent an interesting target for treating biofilm-associated infections. This new methodology is also discussed in Trends Microbiol. (2019, 27(9):728-730).
April 30, 2019
Staphylococcus aureus protein A binding to von Willebrand factor is activated by force
Protein A (SpA) binds to von Willebrand factor (vWF) under flow, but the molecular basis of this stress-dependent interaction has not yet been elucidated. Congrats to Felipe and our collaborators at the University of Pavia who have shown that the SpA-vWF interaction is regulated by a new force-dependent mechanism. This study published in mBio highlights the role of mechanoregulation in controlling the adhesion of S. aureus and shows promise for the design of small inhibitors capable of blocking colonization under high shear stress.
November 1, 2018
Atomic force microscopy-based mechanobiology
Mechanobiology describes how biological systems respond to mechanical stimuli. In a Nature Reviews Physics review paper, together with several experts in the field, the nBio team survey the basic principles, advantages and limitations of applying and combining atomic force microscopy-based modalities with complementary techniques to characterize the morphology, mechanical properties and functional response of complex biological systems to mechanical cues.
September 12, 2018
Bacterial sexuality at the nanoscale
With the Mahillon team we introduce an innovative atomic force microscopy platform to study and mechanically control DNA transfer between single bacteria, focusing on the large conjugative pXO16 plasmid of the Gram-positive bacterium Bacillus thuringiensis, a study published in Nano Lett. This technology may enable researchers to mechanically control gene transfer among a wide range of Gram-positive and Gram-negative bacterial species and to understand the molecular forces involved.
August 14, 2018
Staphylococcus aureus and eczema: a nanoscale view
Bacterium-skin interactions play important roles in skin disorders, yet their molecular details are poorly understood. In a paper published in mBio, we and our collaborators in Ireland decipher the molecular forces at play during adhesion of Staphylococcus aureus to skin corneocytes in the clinically important context of atopic dermatitis, also known as eczema. We identify a unique relationship between the level of natural moisturizing factor in the skin and the strength of bacterium-corneocyte adhesion. Bacterial adhesion is primarily mediated by the surface protein clumping factor B and is enhanced by physical stress, highlighting the role of protein mechanobiology in skin colonization. Similar to a catch bond behavior, this mechanism represents a promising target for the development of novel antistaphylococcal agents.
May 7, 2018
Mechanobiology: Staphylococcus aureus under tension !
Staphylococcus aureus is an important bacterial pathogen which is a leading cause of biofilm-associated infections on indwelling medical devices. Biofilms are currently estimated to be involved in more than 65 % of hospital-acquired infections. There is evidence that bacterial adhesion and biofilm formation are favored under high physical stress, but how this is achieved at the molecular level is not known. In a study published in PNAS, our team, together with the Trinity College Dublin, has elucidated the mechanism by which S. aureus responds to mechanical tension. We focused on the bacterial surface protein ClfA, and on its interaction with fibrinogen, a blood protein that rapidly covers implanted medical devices. Using atomic force microscopy, we showed that ClfA behaves as a force-sensitive molecular switch that potentiates staphylococcal adhesion under mechanical stress. The adhesion of ClfA is weak at low tensile force, but is dramatically enhanced by mechanical tension, as observed with catch bonds. Strong bonds are inhibited by a peptide mimicking Fg, which offers prospects for the development of antiadhesion therapeutics. These findings are of biological significance because they explain at the molecular level the ability of ClfA to promote bacterial attachment under high physiological shear stress. This study emphasizes the role of mechanobiology in staphylococcal biofilms, a topic that we also discusses in a recent perspective article in Science.
For more details, see https://uclouvain.be/fr/sciencetoday/actualites/le-stress-du-staphylocoque-dore.html
April 24, 2018
New ACS Nano paper: Mechanical forces guiding Staphylococcus aureus cellular invasion
Invasion of mammalian cells by S. aureus involves fibronectin-dependent bridging between FnBPs on the bacterial surface and α5β1 integrins in the host cell membrane, but the fundamental forces involved are poorly understood. With our colleagues from the University of Pavia, we have used state of the art single-cell and single-molecule experiments to quantify the molecular forces engaged in this three component interaction, revealing that the fibronectin bridge between FnBPs and the α5β1 integrin is mechanically strong.
March 30, 2018.
New perspective article in Science: Force matters in hospital-acquired infections
Building up on an outstanding paper by Gaub et al. we discuss how extremely strong forces help staphylococci to colonize biomaterials and infect humans.
December 5, 2017.
Physical stress activates the adhesive function of Staphylococcus aureus surface protein clumping factor B
Staphylococcus aureus colonizes the skin and the nose of humans and can cause various disorders, including superficial skin lesions and invasive infections. During nasal colonization, the S. aureus surface protein clumping factor B (ClfB) binds to the squamous epithelial cell envelope protein loricrin, but the molecular interactions involved are poorly understood. In a new paper published in mBio, we unravel the molecular mechanism guiding the ClfB-loricrin interaction. We show that the ClfB-loricrin bond is remarkably strong, consistent with a high affinity "dock, lock and latch" binding mechanism. We discover that the ClfB-loricrin interaction is enhanced under tensile loading, thus providing evidence that the function of a S. aureus surface protein can be activated by physical stress.
September 1-15, 2017.
Pr. Peter Lipke is visiting us
We are honered to host Pr. Peter Lipke (CUNY Brooklyn, USA), world expert in yeast biofilms and longstanding collaborator, for a sabbatical stay. You can see him here learning the basics of AFM with Philippe.
September 5, 2017.
How fibrinogen activates the capture of human plasminogen by staphylococcal surface proteins
Invasive bacterial pathogens can capture host plasminogen and allow it to form plasmin, a process of medical importance as surface-bound plasmin promotes bacterial spreading by cleaving tissue components and favours immune evasion by degrading opsonins. Together with the Speziale and Foster teams, we discovered that Staphylococcus aureus fibronectin-binding proteins bind plasminogen by a sophisticated activation mechanism involving fibrinogen, another ligand found in the blood. The work has just been published in mBio.
June 20, 2017.
Imaging the growth of a bacterial functional amyloid at single-fiber resolution
Amyloids are aggregative protein fibrils best known for their implication in degenerative illnesses such as Alzheimer’s and Parkinson’s diseases. However, microbes can also produce so-called functional amyloids, i.e. amyloids that serve a dedicated biological function. Curli are functional amyloids produced by Escherichia coli as part of the extracellular matrix that holds cells together into biofilms. The molecular events that occur during curli nucleation and fiber extension remain largely unknown. A team of scientists from the VIB lab of Han Remaut (VIB-VUB) and our lab collaborated on a study published in Nat Chem Biol (lien: https://www.nature.com/nchembio/journal/vaop/ncurrent/full/nchembio.2413.html), in which high speed atomic force microscopy was used to film the formation of curli fibers in real-time and at high resolution, revealing new insights into curli nucleation and growth.
April 6, 2017.
New review in Nat Nanotechnol on AFM imaging modes
Together with colleagues in the field, we review the basic principles, advantages and limitations of the most common AFM bioimaging modes, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging. For each of these modes, we discuss recent experiments that highlight their unique capabilities.
March 21, 2017.
Discovery of a peptide capable to prevent biofilm formation by Staphylococcus aureus
S. aureus has an exceptional ability to stick to implanted biomaterials, such as central venous catheters and prosthetic joints, leading to the formation of biofilms. Biofilm-related infections are difficult to eradicate because bacterial cells are protected from host defenses and are resistant to many antibiotics. An attractive alternative to antibiotics is the use of antiadhesion compounds to block bacterial adhesion and biofilm development. In collaboration with Timothy Foster and Joan Geoghegan (Trinity College Dublin), an ISV team identified a peptide capable of preventing the formation of S. aureus biofilms. They first unravelled the molecular interactions by which the surface adhesin SdrC mediates biofilm accumulation. SdrC was found to mediate cell-cell adhesion through weak homophilic bonds, and to also promote strong hydrophobic interaction with inert surfaces. They discovered that a peptide derived from the neuronal cell adhesion molecule β-neurexin is able to inhibit SdrC-dependent attachment to inert surfaces, cell-cell adhesion, and biofilm formation. These findings, published in PNAS raise the possibility that the peptide could be used as a platform for designing a peptidomimetic with potential to prevent biofilm infections.
February 2, 2017.
Congrats to Claire and Valeria for their new paper in ACS Nano.
While it is established that the collagen-binding protein Cna from Staphylococcus aureus binds to collagen via the high-affinity collagen hug mechanism, whether this protein is engaged in other ligand-binding mechanisms is poorly understood. Here, we use atomic force microscopy to demonstrate that Cna mediates attachment to two structurally and functionally different host proteins, i.e., the complement system protein C1q and the extracellular matrix protein laminin, through binding mechanisms that differ from the collagen hug. Both C1q and laminin interactions can be efficiently blocked by monoclonal antibodies directed against the minimal binding domain of Cna.
January 12, 2017.
New ACS Nano perspective article: Microbial Nanoscopy: Breakthroughs, Challenges, and Opportunities.
microbial nanoscopy janvier 2017
October 26, 2016.
Congrats to Philippe and Claire for their new mBio paper.
Together with the Speziale and Foster teams, we unravel the mechanical strength of the Staphylococcus aureus Cna adhesion protein in living bacteria. We show that single Cna-collagen bonds are very strong, reflecting high-affinity binding by the collagen hug mechanism. We discover that the B region behaves as a nanospring capable of sustaining high forces. This unanticipated mechanical response, not previously described for any staphylococcal adhesin, favors a model in which the B region has a mechanical function that is essential for strong ligand binding. Finally, we assess the antiadhesion activity of monoclonal antibodies against Cna, suggesting that they could be used to inhibit S. aureus adhesion.
October 26, 2016.
New review in Nature Microbiol.
Together with the Xiao team from The Johns Hopkins School of Medicine (USA), we discuss the principles, advantages and limitations of the main optical and force nanoscopy techniques available in microbiology, and we highlight some outstanding questions that these new tools may help to answer.
May 10, 2016.
New paper in Nanoscale Horizons.
The human skin is colonized by a wide diversity of microorganisms, including bacterial, fungal and virus species. Although most skin colonizers are harmless or beneficial, some of them like Staphylococcus aureus can be implicated in skin disorders. So far, direct measurement of the molecular forces involved in microbe-skin interactions has not been possible. In a new study with the Staphylococcal research group (Trinity College Dublin, Ireland), we developed a novel nanoscopy technique combining multiparametric atomic force microscopy with single bacterial probes, that enables us to provide spatially-resolved quantitative maps of bacterial–host adhesion on skin surfaces. The method could be used to advance our knowledge of S. aureus adhesion to corneocytes from children with eczema. More broadly, it should find broad utility for studying the molecular basis of host–microbe interactions.
See also news in Chemistryworld: AFM maps bacteria on skin.
April 14, 2016.
New ERC grant NanoStaph.
Yves receives an Advanced Grant from the European Research Council (ERC) to explore staphylococcal biofilms using the new tools of nanotechnology. Staphylococcus aureus is a leading cause of hospital-acquired infections, which are often complicated by the ability of this pathogen to grow as biofilms on indwelling medical devices. Because biofilms protect the bacteria from host defenses and are resistant to many antibiotics, biofilm-related infections are difficult to fight and represent a tremendous burden on our healthcare system. Today, a true molecular understanding of the fundamental interactions driving staphylococcal adhesion and biofilm formation is lacking owing to the lack of high-resolution probing techniques. This knowledge would greatly contribute to the development of novel antiadhesion therapies for combating biofilm infections. This multidisciplinary project aims at developing innovative atomic force microscopy (AFM) techniques in biofilm research, enabling us to understand the molecular mechanisms of S. aureus adhesion in a way that was not possible before, and to optimize the use of anti-adhesion compounds capable to inhibit biofilm formation by this pathogen.