Proteomic & protein analysis by mass spectrometry (MASSPROT)


SYNAPT G2-Si high definition mass spectrometer (WATERS) coupled to the NanoACQUITY UPLC MClass system (WATERS)   Orbitrap Fusion Lumos (IET) coupled with RSLC nanoHPLC Ultimate 3000 (THERMOFISCHER SCIENTIFIC)   Orbitrap Fusion Lumos Nano-Spray Source (IET)  

Technical and scientific expertise

  • Proteomics: scientific and technical advice and support at all stages of sample preparation and mass spectrometry experiments in areas such as:
    • identification and expression changes of proteins
    • identification and quantification of proteins from complex samples
    • localisation of post-translational modifications even in complex samples
    • analysis of intact proteins
  • Bioinformatics: advice and data analysis using appropriate IT tools and software

Main equipment

Mass spectrometry, chromatography, two-dimensional electrophoresis techniques:

  • Orbitrap Fusion Lumos Tribrid Mass spectrometer (Thermo Scientific)
  • Orbitrap Exploris 240 Mass spectrometer (Thermo Scientific)
  • Synapt G2si spectrometer (Waters)
  • Acquity MClass nano UPLC System (Waters)
  • Ultimate 3000 RSLN nano HPLC System (Thermo Scientific)

Get an overview of the platform’s facilities via a virtual tour.


  • Measurement of intact protein masses for quality control
  • Identification of purified proteins on gel
  • De novo sequencing of peptides for protein identification
  • Quantification of differential protein expression
  • Identification of covalent modification sites by peptide fragmentation/dissociation

Services available to

  • UCLouvain students and researchers
  • Non-UCLouvain parties


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