Overview of technologies
1D and 2D-PAGE 
- Classical (silver, Colloidal Coomassie blue)
- Fluorescent protein stains (DIGE using CyTM dyes)
- Post translational modification specific (eg Pro-Q Diamond)
- Gel Image Analysis
- Image scanner Labscan
- Image Master 2D Platinum
- Decyder v7.0
- Spot Excision/ Digestion of Proteins
Mass Spectrometric analysis
- Peptide mass fingerprinting MALDI TOF MS
- Peptide fragments fingerprint ESI-IT
- Sequence analysis 4800 MALDI TOF/TOF
- Post-translation modification analysis-phosphorylation, acetylation
- iTRAQ and LC-MS/MS workflows for protein identification and relative quantification
- Bioinformatics
- Massspectrometry data analysis
- Data storage
Applications
- Measurement of masses of intact proteins for quality control
- Gel-purified protein identification by peptide mass fingerprinting and peptide fragmentation
- De Novo sequencing of peptides for protein identification
- Quantification of differential protein expression by 2D-differential gel electrophoresis (DIGE)
- Quantification of differential protein expression by gel-free proteomics and 2D-LC-MS (iTraq, ICAT...)
- Identification of sites of covalent modification by collision-induced dissociation (CID) fragmentation of peptides or by electron transfer dissociation of peptides (ETD)