Bioactive lipids are important molecular mediators in inflammatory settings. As inflammation, and especially chronic inflammation, are important drivers of many chronic disorders, investigating the role of lipid mediators in inflammation could have a strong impact. Thus, our group aims to identify novel lipid mediators and lipid-related targets (i.e. receptors and enzymes) in inflammatory settings. Bioactive lipids are selected either based on reported effects or following their identification in lipidomics studies performed in our laboratory. The effects of these bioactive lipids are assessed, in vitro, ex vivo and in vivo, to determine their potential impact on inflammation (Figure 1). Once interesting lipids are selected and their effects identified, we turn to the identification of potential means to control their effects in vivo, for example by using agonists or antagonists of their receptors, or interfering with their metabolic pathways using pharmacological tools. A key aspect of our research strategy is to integrate the information gathered by quantifying the lipids and the information obtained by assessing their effects in our models. Over the years, this strategy allowed us to put forth several lipids and enzymes as important mediators of inflammation.