Cell Analysis (BD FACSCanto II + BD FACSCalibur)
- Multiparametric Immunophenotype and rare events detection
- TCR repertoire
- Signal Transduction (PhosFlow)
- Calcium Flux
- Cell cycle
- DNA/RNA content Fluorescent in-situ hybridization (Flow-FISH) to quantify the telomeres length
- Cell proliferation
- Cell death
- Mitochondria and cellular membrane potential alterations
- mRNA and micrORNa getection
- Exosomes and microvesicles detection
Cell Sorting (BD FACSArialll)
- Multiparametric isolation of cells from mixed populations, stem cells, rare events
- Single-cell sorting for clonogenic assays Single-cell cloning of hybridoma cells for monoclonal antibodies production
- Selection of transfected cells with tags and expression markers
- Protein interaction study with FRET technique using the 561m laser
Standard analyses
- T, B, NK, NKT, p/mDC, Neutrophils, Eosinophils, Basophils, monocytes, T-regs, Th1, Th2, Th17, 18 cells
CD45,CD3,CD4, CD8,CD16/CD56,CD19,CD20,CD123
CD14,CD11c,CD25,CD183,CD185,CD194,CD196 - Naive, central memory, effectors cells
CD45RA,CD27,CCR7,CD28,CD62L,|gD/M - Exhaustion
PD-1, CTLA-4, TIM3, CD57, CD38 - Apoptosis
AnnexinV/PI, JC-1 - ROS
DCFDA, MitoSOX - Phosphorylation
- Proliferation
CFSE, Ki-67
Specific analysis on demand
Available equipments
- FACSCalibur: 405, 488, 633
- FACSCantoll: 488,633
- FACSArialli: 405, 488, 561, 633
- GentleMACS Tissue Dissociator
- DIVA and FlowJo softwares
Contact
Davide brusa, PhD: davide.brusa@ucouvain.be
Damien Gheldof, PhD: damien.gheldof@uclouvain.be
Institute of Experimental and Clinical Research REC)
Universite catholique de Louvain UGL
Avenue hippocrate, 55 ote 31.55.20 155+2 - 1200 Brussels -
BELGIUM
Office: 32.2.764.55.63
Lab : 32.2.764.55.68
https://uclouvain.be/en/research-institutes/irec/cytometrie-de-flux.html