A propos

CYTOFLUX

 

 

Cell Analysis (BD FACSCanto II + BD FACSCalibur)

  • Multiparametric Immunophenotype and rare events detection
  • TCR repertoire
  • Signal Transduction (PhosFlow)
  • Calcium Flux
  • Cell cycle
  • DNA/RNA content Fluorescent in-situ hybridization (Flow-FISH) to quantify the telomeres length
  • Cell proliferation
  • Cell death
  • Mitochondria and cellular membrane potential alterations
  • mRNA and micrORNa getection
  • Exosomes and microvesicles detection

 

Cell Sorting (BD FACSArialll)

  • Multiparametric isolation of cells from mixed populations, stem cells, rare events
  • Single-cell sorting for clonogenic assays Single-cell cloning of hybridoma cells for monoclonal antibodies production
  • Selection of transfected cells with tags and expression markers
  • Protein interaction study with FRET technique using the 561m laser

Standard analyses

  • T, B, NK, NKT, p/mDC, Neutrophils, Eosinophils, Basophils, monocytes, T-regs, Th1, Th2, Th17, 18 cells
    CD45,CD3,CD4, CD8,CD16/CD56,CD19,CD20,CD123
    CD14,CD11c,CD25,CD183,CD185,CD194,CD196
  • Naive, central memory, effectors cells
    CD45RA,CD27,CCR7,CD28,CD62L,|gD/M
  • Exhaustion
    PD-1, CTLA-4, TIM3, CD57, CD38
  • Apoptosis
    AnnexinV/PI, JC-1
  • ROS
    DCFDA, MitoSOX
  • Phosphorylation
  • Proliferation
    CFSE, Ki-67

Specific analysis on demand

Available equipments

  • FACSCalibur: 405, 488, 633
  • FACSCantoll: 488,633
  • FACSArialli: 405, 488, 561, 633
  • GentleMACS Tissue Dissociator
  • DIVA and FlowJo softwares

Contact

Davide brusa, PhD: davide.brusa@ucouvain.be
Damien Gheldof, PhD: damien.gheldof@uclouvain.be

Institute of Experimental and Clinical Research REC)
Universite catholique de Louvain UGL
Avenue hippocrate, 55 ote 31.55.20 155+2 - 1200 Brussels -
BELGIUM
Office: 32.2.764.55.63
Lab : 32.2.764.55.68
https://uclouvain.be/en/research-institutes/irec/cytometrie-de-flux.html