Dynamic Nuclear Polarization
Hyperpolarization allows to considerably increase the sensitivity (>10.000) of 13C-NMR spectroscopy. Our Hypersense (Oxford Instruments) system, used in combination with the Bruker Biospec 11.7T MRI system (for in-vivo application) or the with Bruker Ascend 600MHz NMR system (for in-vitro applications), is able to hyperpolarize 13C-enriched substrates for the monitoring of metabolic fluxes in real time. The detection of the metabolites is performed using 13C-MRS coils.
The follow-up of different metabolic fluxes, such as the 13C-pyruvate to 13C-lactate (or 13C- alanine) exchange allows the monitoring of tumor metabolism and glycolysis.