Electron Paramagnetic Resonance & Electron Paramagnetic Resonance Imaging


Electron Paramagnetic Resonance

Electron paramagnetic resonance spectroscopy/imaging is the gold standard method for detecting and quantifying free radicals in biological media. The NEST platform offers access to the following equipment:

  • X-band EPR spectrometer (Bruker EMX+, 9 GHz) for in vitro applications
  • Benchtop X-band EPR spectrometer (Magnettech Miniscope, 9 GHz for in vitro applications
  • L-band (1 GHz) and X-band (9 GHz) imaging EPR system (Bruker Elexys) for in vitro and in vivo applications
  • L-band EPR spectrometer (Magnettech, 1 GHz) for in vivo applications (small animals)
  • Clinical L-band EPR spectrometer (whole body, 1 GHz) for human studies


EPR applications include (non-exhaustive list):

  • Free radicals measurements and characterization by spin trapping
  • Quantification of melanin / melanoma cells in tissues
  • Molecular dynamics, microviscosity, micropolarity in tissues and drug delivery systems
  • Dosimetry (retrospective dosimetry in bones and teeth)
  • Dosimetry in phantoms (external beams, brachytherapy)
  • Tissue oxygenation, Oxygen consumption
  • Redox status, pH
  • Superparamagnetic iron oxides nanoparticles (SPIO) quantification