Welcome to 2IP optical imaging platform
The 2IP multi-user platform was created in 2011 thanks to the sharing of imaging equipment present among the IREC research groups. 2IP gradually acquires new equipment and is now recognized as a UCL platform. 2IP is composed of one research logisticians (Caroline Bouzin) and two technicians (Michele de Beukelaer and Aurélie Daumerie).
2IP offers access to:
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Sample preparation services:
Equipment: three microtomes (Microm HM340E, including STS and Coolcut), one cryostat (Microm HM560) and a coverslipper (Sakura)
Techniques: paraffin and cryo-sectioning, histological staining and immunostaining (including TSA multiplex)
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Image acquisition devices:
Equipment: two high throughput brightfield slide scanners (Leica SCN400 and 3DHistech ScanII NEW)
a high throughput fluorescence, brightfield & polarized light slide scanner configured for multiplex imaging (7 colors) (Zeiss Axioscan.z1)
an upright fluorescence (4 colors) microscope equipped with an ApoTome module (Zeiss AxioImager.z1/ApoTome1)
an inverted confocal microscope equipped with 4 lasers, VSDs, 2 GaAsp and 1 Airyscan detectors, allowing fixed and live samples imaging (Zeiss LSM800)
a lightsheet fluorescence microscope for 3D imaging equipped with 4 lasers and 2 cameras, allowing fixed/cleared or live samples imaging (Zeiss Lightsheet.z1)
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Image analysis support:
Software:
Visiopharm Author (2 licences and dedicated workstations)
Indicalabs Halo (1 licence and dedicated workstation)
Arivis4D (1 licence and dedicated workstation)
ZEN (diverse modules: image analysis, co-localization, spectral unmixing, FRET, FRAP,...)
ImageJ/Fiji (open source)
QuPath (open source)