Would you like to contribute to cutting-edge projects in basic and clinical research?
Are you motivated by learning new technologies related to histology and imaging?
Join the highly motivated team at IREC's imaging platform (2IP)!
Job reference: SF 36412
Welcome to 2IP optical imaging platform - RRID:SCR_023378
The 2IP multi-user platform was created in 2011 thanks to the sharing of imaging equipment present among the IREC research groups. 2IP gradually acquires new equipment and is now recognized as a UCL platform. 2IP is composed of one research logisticians and two technicians.
2IP offers access to:
Sample preparation services :
Equipment:
Three microtomes (Microm HM340E, including STS and Coolcut), one cryostat (Microm HM560) and a coverslipper (Sakura)
Techniques:
Paraffin and cryo-sectioning, histological staining and immunostaining (including TSA multiplex)
Image acquisition devices:
Equipment:
- Two high throughput brightfield slide scanners (Leica SCN400 and 3DHistech ScanII)
- A high throughput fluorescence, brightfield & polarized light slide scanner configured for multiplex imaging (7 colors) (Zeiss Axioscan.z1)
- An upright fluorescence (4 colors) microscope equipped with an ApoTome module (Zeiss AxioImager.z1/ApoTome1)
- An inverted confocal microscope equipped with 4 lasers, VSDs, 2 GaAsp and 1 Airyscan detectors, allowing fixed and live samples imaging (Zeiss LSM800)
- A lightsheet fluorescence microscope for 3D imaging equipped with 4 lasers and 2 cameras, allowing fixed/cleared or live samples imaging (Zeiss Lightsheet.z1)
Image analysis support:
Software:
- Visiopharm Author (2 licences and dedicated workstations)
- Indicalabs Halo (1 licence and dedicated workstation)
- Arivis4D (1 licence and dedicated workstation)
- ZEN (diverse modules: image analysis, co-localization, spectral unmixing, FRET, FRAP,...)
- ImageJ/Fiji (open source)
- QuPath (open source)