Imaging Platform

2IP

 

Welcome to 2IP optical imaging platform

The 2IP multi-user platform was created in 2011 thanks to the sharing of imaging equipment present among the IREC research groups. 2IP gradually acquires new equipment and is now recognized as a UCL platform. 2IP is composed of one research logisticians (Caroline Bouzin) and two technicians (Michele de Beukelaer and Aurélie Daumerie).
 

2IP offers access to:

  •      Sample preparation services:

Equipment: three microtomes (Microm HM340E, including STS and Coolcut NEW), one cryostat (Microm HM560) and a coverslipper (Sakura)

Techniques: paraffin and cryo-sectioning, histological staining and immunostaining (including TSA multiplex)

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  •      Image acquisition devices:

Equipmenta high throughput brightfield slide scanner (Leica SCN400)

   a high throughput fluorescence, brightfield & polarized light slide scanner configured for multiplex imaging (7 colors) (Zeiss Axioscan.z1) NEW

   an upright fluorescence (4 colors) microscope equipped with an ApoTome module (Zeiss AxioImager.z1/ApoTome1)

   an inverted confocal microscope equipped with 4 lasers, VSDs, 2 GaAsp and 1 Airyscan detectors, allowing fixed and live samples imaging (Zeiss LSM800)

   a lightsheet fluorescence microscope for 3D imaging equipped with 4 lasers and 2 cameras, allowing fixed/cleared or live samples imaging (Zeiss Lightsheet.z1)

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  •     Image analysis support:

Software:

Visiopharm Author (2 licences and dedicated workstations)

Indicalabs Halo (1 licence and dedicated workstation) NEW

Arivis4D (1 licence and dedicated workstation)

ZEN (diverse modules: image analysis, co-localization, spectral unmixing, FRET, FRAP,...)

ImageJ/Fiji (open source)

QuPath (open source) NEW

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Platform charter