Whole Slide Imaging (WSI) technology (or virtual microscopy) consists of scanning histological sections at high resolution, in brightfield or in fluorescence, in order to obtain an overview of the biological tissue. In addition to the global vision of the histological sections, it is possible to move on the virtual slide, to adjust the brightness and to zoom in on areas of interest with high definition.
The main advantage over traditional microscopy images (pathologists or researchers selecting fields of interest) is to make the analysis independent of the choices made by the first observer. Furthermore, the comparison of images, for example of different stainings performed on adjacent sections, is greatly facilitated since the images can be placed side by side on the same computer screen and synchronized.
Digital images of whole sections can be viewed and stored, but also shared, thus facilitating collaborative studies, teaching and publication. In addition, the content of digital images can be exploited in a standardized and rapid manner using specific software for quantitative measurement of stained areas, cell number, biomarkers, vascularization, fibrosis, etc.
| Brightfield slide scanner | Fluorescence slide scanner |
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Pannoramic ScanII  |
Axioscan.z1   |
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This slide scanner allows high throughput (150 slides) whole slide imaging in brightfield at very good resolution. |
This slide scanner allows high throughput (100 slides) whole slide imaging in brightfield, polarized light and multi-channel fluorescence up to 40x magnification. |
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User guide tutorials: ZEN Knowledge Base
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Image format .mrxs Visualization via 3DHistech SlideViewer or Cytomine (centralized storage and multi-user access via web) or QuPath (possibility of quantification) |
Image format .czi Visualization via ZEN Lite ou QuPath (possibility of quantification) |
