Viral genome research by PCR DNA HIV

MBLG

What tests do we perform?

Follow-up of children born to HIV-1 infected mothers
Measured parameters
- DNA Presence of HIV-1 genome :

  • QIAamp DNA Blood Mini Kit
  • MAXIMA SYBR Green/Rox qPCR Master

Follow-up of children born to HIV-2 infected mothers
Measured parameters
- DNA Presence of HIV-2 genome :

  • QIAamp DNA Blood Mini Kit
  • Digital PCR (ddPCR, QX 200 Bio-Rad)

Sample compliance

Volume required: 2 ml of blood collected on EDTA

Samples cannot be :
- be hemolyzed
- be centrifuged

For provirus research (DNA PCR)
Samples are collected on EDTA:
- after collection, the entire blood sample must arrive at the laboratory within 4 days at room temperature.
NB: if delay > 4 days, send a plasma* in addition

For viral RNA testing (viral load by RNA RT-PCR)
- After centrifugation, the plasma must arrive at the laboratory within 24 hours at room temperature, within 5 days in the refrigerator or frozen.
NB: use polypropylene tubes resistant to -80°C with screw cap

Request forms
- FO(CLIN)04 Child follow-up (In French)
- FO(CLIN)07 Opvolging van kinderen

Response time
7 working days